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capmeter 6  (MathWorks Inc)


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    MathWorks Inc capmeter 6
    Non-SG fusion in whole-cell recording is wortmannin/adenosine insensitive. (A) Typical whole-cell <t>capacitance</t> records and composite statistics for RBL cells during cytoplasmic infusion of 200 μM Ca with control cytoplasmic solution (top) and with 5 μM wortmannin and 0.5 mM adenosine (bottom). Differences are not significant. (B) Exchange current densities (top) and capacitance responses (bottom) for BHK cells in which membrane fusion was activated by outward Na/Ca exchange current with control cytoplasmic solution (left bar graph), with 0.5 mM adenosine (middle bar graph), and with 5 μM wortmannin and 0.5 mM adenosine (right bar graph) are shown.
    Capmeter 6, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/capmeter 6/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    capmeter 6 - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Ca-dependent Nonsecretory Vesicle Fusion in a Secretory Cell"

    Article Title: Ca-dependent Nonsecretory Vesicle Fusion in a Secretory Cell

    Journal: The Journal of General Physiology

    doi: 10.1085/jgp.200709950

    Non-SG fusion in whole-cell recording is wortmannin/adenosine insensitive. (A) Typical whole-cell capacitance records and composite statistics for RBL cells during cytoplasmic infusion of 200 μM Ca with control cytoplasmic solution (top) and with 5 μM wortmannin and 0.5 mM adenosine (bottom). Differences are not significant. (B) Exchange current densities (top) and capacitance responses (bottom) for BHK cells in which membrane fusion was activated by outward Na/Ca exchange current with control cytoplasmic solution (left bar graph), with 0.5 mM adenosine (middle bar graph), and with 5 μM wortmannin and 0.5 mM adenosine (right bar graph) are shown.
    Figure Legend Snippet: Non-SG fusion in whole-cell recording is wortmannin/adenosine insensitive. (A) Typical whole-cell capacitance records and composite statistics for RBL cells during cytoplasmic infusion of 200 μM Ca with control cytoplasmic solution (top) and with 5 μM wortmannin and 0.5 mM adenosine (bottom). Differences are not significant. (B) Exchange current densities (top) and capacitance responses (bottom) for BHK cells in which membrane fusion was activated by outward Na/Ca exchange current with control cytoplasmic solution (left bar graph), with 0.5 mM adenosine (middle bar graph), and with 5 μM wortmannin and 0.5 mM adenosine (right bar graph) are shown.

    Techniques Used: Control, Membrane

    Non-SG fusion in whole-cell recordings is strongly inhibited by cell swelling. (A and B) Bar graphs give exchange current densities (top) and capacitance responses (bottom) for two batches of BHK cells in which membrane fusion was activated by outward Na/Ca exchange current. (A) Effects of cytoplasmic osmolarity on fusion responses. The left bar graphs give results for isoosmotic solution, the middle bars for cytoplasmic solution with 200 mM sucrose, and the right bars for cytoplasmic solution diluted 30% with distilled water. (B) Effects of extracellular osmolarity on fusion responses. From left to right the bar graphs are with (1) standard extracellular solution for 2 min after cell opening, (2) standard extracellular solution with the NMG-aspartate concentration reduced by 80 mM for 2 min after cell opening, (3) standard solution reapplied for 2 min after applying hypoosmotic solution for 2 min, and (4), as in (3) with 5 μM wortmannin in all cytoplasmic solutions. (C) Capacitance responses of RBL cells for pipette perfusion of cytoplasmic solution with 200 μM free Ca. The left bar graph indicates the response magnitude for control cells, the middle graph for cells swollen with hyperosmotic cytoplasmic solution (200 mM sucrose) for 2 min, and the right bar for cells swollen with extracellular solution in which the NMG concentration was reduced by 80 mM.
    Figure Legend Snippet: Non-SG fusion in whole-cell recordings is strongly inhibited by cell swelling. (A and B) Bar graphs give exchange current densities (top) and capacitance responses (bottom) for two batches of BHK cells in which membrane fusion was activated by outward Na/Ca exchange current. (A) Effects of cytoplasmic osmolarity on fusion responses. The left bar graphs give results for isoosmotic solution, the middle bars for cytoplasmic solution with 200 mM sucrose, and the right bars for cytoplasmic solution diluted 30% with distilled water. (B) Effects of extracellular osmolarity on fusion responses. From left to right the bar graphs are with (1) standard extracellular solution for 2 min after cell opening, (2) standard extracellular solution with the NMG-aspartate concentration reduced by 80 mM for 2 min after cell opening, (3) standard solution reapplied for 2 min after applying hypoosmotic solution for 2 min, and (4), as in (3) with 5 μM wortmannin in all cytoplasmic solutions. (C) Capacitance responses of RBL cells for pipette perfusion of cytoplasmic solution with 200 μM free Ca. The left bar graph indicates the response magnitude for control cells, the middle graph for cells swollen with hyperosmotic cytoplasmic solution (200 mM sucrose) for 2 min, and the right bar for cells swollen with extracellular solution in which the NMG concentration was reduced by 80 mM.

    Techniques Used: Membrane, Concentration Assay, Transferring, Control

    Method to determine whole-cell capacitance via square wave perturbation. For whole-cell recording from cells with time constants >50 μs, square pulses were employed at 0.5 kHz with an amplitude of 20 mV. Model current is shown in A. Peak current, a , and the projected steady-state current, b , were determined as described in Materials and methods. (B) Half of the current from a typical recording (dots) with the fitted exponential function used to determine cell parameters is shown. The asymptote of current was determined using the averages of three equally spaced data sections (dashed sections) according to , given in Materials and methods. The asymptote was subtracted and the data range from the peak to a point located at ∼3τ, estimated as peak current times e −3 , was used to determine the exponential constants via linear regression of the log of the decaying current transient (solid line).
    Figure Legend Snippet: Method to determine whole-cell capacitance via square wave perturbation. For whole-cell recording from cells with time constants >50 μs, square pulses were employed at 0.5 kHz with an amplitude of 20 mV. Model current is shown in A. Peak current, a , and the projected steady-state current, b , were determined as described in Materials and methods. (B) Half of the current from a typical recording (dots) with the fitted exponential function used to determine cell parameters is shown. The asymptote of current was determined using the averages of three equally spaced data sections (dashed sections) according to , given in Materials and methods. The asymptote was subtracted and the data range from the peak to a point located at ∼3τ, estimated as peak current times e −3 , was used to determine the exponential constants via linear regression of the log of the decaying current transient (solid line).

    Techniques Used:

    Amperometric and capacitance measurement in RBL cells. (A) Schematic illustration of the intra-patch pipette carbon electrode. Carbon electrodes were prepared to allow facile insertion and manipulation in the patch pipette holder employed. (B) In the cell-attached configuration, 2 μM of calcium ionophore, A23187, triggers profuse exocytosis. Two distinct vesicle pools are observed. One is the secretory granule (SG) pool with large-amplitude capacitance steps and amperometric spikes upon stimulation. The other pool (at the end of the trace) contains vesicles of much smaller size that do not release serotonin (non-SG pool). (C) Amperometric recording of SG fusion in an ∼20 μm (diameter) excised patch. (D) Expansion of C. In most cases, SGs are lost from membrane patches during the excision procedure. Occasionally, when SGs are preserved, fusion gives rise to capacitance steps of tens of fF, indicating that the diameter of the granules is close to micrometer range. A typical fusion event with fusion pore dilation is marked between two broken lines. A gradual increase of capacitance, transient increase of conductance, and the amperometric foot-signal is observed. Here and in all subsequent figures, numbers given between axis ticks indicate the tick interval.
    Figure Legend Snippet: Amperometric and capacitance measurement in RBL cells. (A) Schematic illustration of the intra-patch pipette carbon electrode. Carbon electrodes were prepared to allow facile insertion and manipulation in the patch pipette holder employed. (B) In the cell-attached configuration, 2 μM of calcium ionophore, A23187, triggers profuse exocytosis. Two distinct vesicle pools are observed. One is the secretory granule (SG) pool with large-amplitude capacitance steps and amperometric spikes upon stimulation. The other pool (at the end of the trace) contains vesicles of much smaller size that do not release serotonin (non-SG pool). (C) Amperometric recording of SG fusion in an ∼20 μm (diameter) excised patch. (D) Expansion of C. In most cases, SGs are lost from membrane patches during the excision procedure. Occasionally, when SGs are preserved, fusion gives rise to capacitance steps of tens of fF, indicating that the diameter of the granules is close to micrometer range. A typical fusion event with fusion pore dilation is marked between two broken lines. A gradual increase of capacitance, transient increase of conductance, and the amperometric foot-signal is observed. Here and in all subsequent figures, numbers given between axis ticks indicate the tick interval.

    Techniques Used: Transferring, Membrane

    Non-SG fusion is not blocked by antibodies against PI(3)P and PI(4)P. In whole-cell recording, antibodies (1:100 dilution) were added to the cytoplasmic (pipette) solution, which was dialyzed into cells for 5 min before Ca 2+ was infused to trigger fusion. (A) A typical record from an RBL cell. The Ca 2+ -activated conductance rise was used to define the t 0 point. (B) The rise of capacitance was well described by the delayed monoexponential function given in the figure. The variable k 2 is the rate constant used for statistical analysis. The number of the data points was reduced to highlight the fitted curve. Antibodies against PI(3)P (C), PI(4)P (D), and both (E) fail to block non-SG fusion.
    Figure Legend Snippet: Non-SG fusion is not blocked by antibodies against PI(3)P and PI(4)P. In whole-cell recording, antibodies (1:100 dilution) were added to the cytoplasmic (pipette) solution, which was dialyzed into cells for 5 min before Ca 2+ was infused to trigger fusion. (A) A typical record from an RBL cell. The Ca 2+ -activated conductance rise was used to define the t 0 point. (B) The rise of capacitance was well described by the delayed monoexponential function given in the figure. The variable k 2 is the rate constant used for statistical analysis. The number of the data points was reduced to highlight the fitted curve. Antibodies against PI(3)P (C), PI(4)P (D), and both (E) fail to block non-SG fusion.

    Techniques Used: Transferring, Blocking Assay



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    capmeter 6  (MathWorks Inc)
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    MathWorks Inc capmeter 6
    Non-SG fusion in whole-cell recording is wortmannin/adenosine insensitive. (A) Typical whole-cell <t>capacitance</t> records and composite statistics for RBL cells during cytoplasmic infusion of 200 μM Ca with control cytoplasmic solution (top) and with 5 μM wortmannin and 0.5 mM adenosine (bottom). Differences are not significant. (B) Exchange current densities (top) and capacitance responses (bottom) for BHK cells in which membrane fusion was activated by outward Na/Ca exchange current with control cytoplasmic solution (left bar graph), with 0.5 mM adenosine (middle bar graph), and with 5 μM wortmannin and 0.5 mM adenosine (right bar graph) are shown.
    Capmeter 6, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/capmeter 6/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    capmeter 6 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Non-SG fusion in whole-cell recording is wortmannin/adenosine insensitive. (A) Typical whole-cell capacitance records and composite statistics for RBL cells during cytoplasmic infusion of 200 μM Ca with control cytoplasmic solution (top) and with 5 μM wortmannin and 0.5 mM adenosine (bottom). Differences are not significant. (B) Exchange current densities (top) and capacitance responses (bottom) for BHK cells in which membrane fusion was activated by outward Na/Ca exchange current with control cytoplasmic solution (left bar graph), with 0.5 mM adenosine (middle bar graph), and with 5 μM wortmannin and 0.5 mM adenosine (right bar graph) are shown.

    Journal: The Journal of General Physiology

    Article Title: Ca-dependent Nonsecretory Vesicle Fusion in a Secretory Cell

    doi: 10.1085/jgp.200709950

    Figure Lengend Snippet: Non-SG fusion in whole-cell recording is wortmannin/adenosine insensitive. (A) Typical whole-cell capacitance records and composite statistics for RBL cells during cytoplasmic infusion of 200 μM Ca with control cytoplasmic solution (top) and with 5 μM wortmannin and 0.5 mM adenosine (bottom). Differences are not significant. (B) Exchange current densities (top) and capacitance responses (bottom) for BHK cells in which membrane fusion was activated by outward Na/Ca exchange current with control cytoplasmic solution (left bar graph), with 0.5 mM adenosine (middle bar graph), and with 5 μM wortmannin and 0.5 mM adenosine (right bar graph) are shown.

    Article Snippet: Capacitance measurement software, Capmeter 6, was developed in MATLAB using its data acquisition toolbox (R2006b; The MathWorks).

    Techniques: Control, Membrane

    Non-SG fusion in whole-cell recordings is strongly inhibited by cell swelling. (A and B) Bar graphs give exchange current densities (top) and capacitance responses (bottom) for two batches of BHK cells in which membrane fusion was activated by outward Na/Ca exchange current. (A) Effects of cytoplasmic osmolarity on fusion responses. The left bar graphs give results for isoosmotic solution, the middle bars for cytoplasmic solution with 200 mM sucrose, and the right bars for cytoplasmic solution diluted 30% with distilled water. (B) Effects of extracellular osmolarity on fusion responses. From left to right the bar graphs are with (1) standard extracellular solution for 2 min after cell opening, (2) standard extracellular solution with the NMG-aspartate concentration reduced by 80 mM for 2 min after cell opening, (3) standard solution reapplied for 2 min after applying hypoosmotic solution for 2 min, and (4), as in (3) with 5 μM wortmannin in all cytoplasmic solutions. (C) Capacitance responses of RBL cells for pipette perfusion of cytoplasmic solution with 200 μM free Ca. The left bar graph indicates the response magnitude for control cells, the middle graph for cells swollen with hyperosmotic cytoplasmic solution (200 mM sucrose) for 2 min, and the right bar for cells swollen with extracellular solution in which the NMG concentration was reduced by 80 mM.

    Journal: The Journal of General Physiology

    Article Title: Ca-dependent Nonsecretory Vesicle Fusion in a Secretory Cell

    doi: 10.1085/jgp.200709950

    Figure Lengend Snippet: Non-SG fusion in whole-cell recordings is strongly inhibited by cell swelling. (A and B) Bar graphs give exchange current densities (top) and capacitance responses (bottom) for two batches of BHK cells in which membrane fusion was activated by outward Na/Ca exchange current. (A) Effects of cytoplasmic osmolarity on fusion responses. The left bar graphs give results for isoosmotic solution, the middle bars for cytoplasmic solution with 200 mM sucrose, and the right bars for cytoplasmic solution diluted 30% with distilled water. (B) Effects of extracellular osmolarity on fusion responses. From left to right the bar graphs are with (1) standard extracellular solution for 2 min after cell opening, (2) standard extracellular solution with the NMG-aspartate concentration reduced by 80 mM for 2 min after cell opening, (3) standard solution reapplied for 2 min after applying hypoosmotic solution for 2 min, and (4), as in (3) with 5 μM wortmannin in all cytoplasmic solutions. (C) Capacitance responses of RBL cells for pipette perfusion of cytoplasmic solution with 200 μM free Ca. The left bar graph indicates the response magnitude for control cells, the middle graph for cells swollen with hyperosmotic cytoplasmic solution (200 mM sucrose) for 2 min, and the right bar for cells swollen with extracellular solution in which the NMG concentration was reduced by 80 mM.

    Article Snippet: Capacitance measurement software, Capmeter 6, was developed in MATLAB using its data acquisition toolbox (R2006b; The MathWorks).

    Techniques: Membrane, Concentration Assay, Transferring, Control

    Method to determine whole-cell capacitance via square wave perturbation. For whole-cell recording from cells with time constants >50 μs, square pulses were employed at 0.5 kHz with an amplitude of 20 mV. Model current is shown in A. Peak current, a , and the projected steady-state current, b , were determined as described in Materials and methods. (B) Half of the current from a typical recording (dots) with the fitted exponential function used to determine cell parameters is shown. The asymptote of current was determined using the averages of three equally spaced data sections (dashed sections) according to , given in Materials and methods. The asymptote was subtracted and the data range from the peak to a point located at ∼3τ, estimated as peak current times e −3 , was used to determine the exponential constants via linear regression of the log of the decaying current transient (solid line).

    Journal: The Journal of General Physiology

    Article Title: Ca-dependent Nonsecretory Vesicle Fusion in a Secretory Cell

    doi: 10.1085/jgp.200709950

    Figure Lengend Snippet: Method to determine whole-cell capacitance via square wave perturbation. For whole-cell recording from cells with time constants >50 μs, square pulses were employed at 0.5 kHz with an amplitude of 20 mV. Model current is shown in A. Peak current, a , and the projected steady-state current, b , were determined as described in Materials and methods. (B) Half of the current from a typical recording (dots) with the fitted exponential function used to determine cell parameters is shown. The asymptote of current was determined using the averages of three equally spaced data sections (dashed sections) according to , given in Materials and methods. The asymptote was subtracted and the data range from the peak to a point located at ∼3τ, estimated as peak current times e −3 , was used to determine the exponential constants via linear regression of the log of the decaying current transient (solid line).

    Article Snippet: Capacitance measurement software, Capmeter 6, was developed in MATLAB using its data acquisition toolbox (R2006b; The MathWorks).

    Techniques:

    Amperometric and capacitance measurement in RBL cells. (A) Schematic illustration of the intra-patch pipette carbon electrode. Carbon electrodes were prepared to allow facile insertion and manipulation in the patch pipette holder employed. (B) In the cell-attached configuration, 2 μM of calcium ionophore, A23187, triggers profuse exocytosis. Two distinct vesicle pools are observed. One is the secretory granule (SG) pool with large-amplitude capacitance steps and amperometric spikes upon stimulation. The other pool (at the end of the trace) contains vesicles of much smaller size that do not release serotonin (non-SG pool). (C) Amperometric recording of SG fusion in an ∼20 μm (diameter) excised patch. (D) Expansion of C. In most cases, SGs are lost from membrane patches during the excision procedure. Occasionally, when SGs are preserved, fusion gives rise to capacitance steps of tens of fF, indicating that the diameter of the granules is close to micrometer range. A typical fusion event with fusion pore dilation is marked between two broken lines. A gradual increase of capacitance, transient increase of conductance, and the amperometric foot-signal is observed. Here and in all subsequent figures, numbers given between axis ticks indicate the tick interval.

    Journal: The Journal of General Physiology

    Article Title: Ca-dependent Nonsecretory Vesicle Fusion in a Secretory Cell

    doi: 10.1085/jgp.200709950

    Figure Lengend Snippet: Amperometric and capacitance measurement in RBL cells. (A) Schematic illustration of the intra-patch pipette carbon electrode. Carbon electrodes were prepared to allow facile insertion and manipulation in the patch pipette holder employed. (B) In the cell-attached configuration, 2 μM of calcium ionophore, A23187, triggers profuse exocytosis. Two distinct vesicle pools are observed. One is the secretory granule (SG) pool with large-amplitude capacitance steps and amperometric spikes upon stimulation. The other pool (at the end of the trace) contains vesicles of much smaller size that do not release serotonin (non-SG pool). (C) Amperometric recording of SG fusion in an ∼20 μm (diameter) excised patch. (D) Expansion of C. In most cases, SGs are lost from membrane patches during the excision procedure. Occasionally, when SGs are preserved, fusion gives rise to capacitance steps of tens of fF, indicating that the diameter of the granules is close to micrometer range. A typical fusion event with fusion pore dilation is marked between two broken lines. A gradual increase of capacitance, transient increase of conductance, and the amperometric foot-signal is observed. Here and in all subsequent figures, numbers given between axis ticks indicate the tick interval.

    Article Snippet: Capacitance measurement software, Capmeter 6, was developed in MATLAB using its data acquisition toolbox (R2006b; The MathWorks).

    Techniques: Transferring, Membrane

    Non-SG fusion is not blocked by antibodies against PI(3)P and PI(4)P. In whole-cell recording, antibodies (1:100 dilution) were added to the cytoplasmic (pipette) solution, which was dialyzed into cells for 5 min before Ca 2+ was infused to trigger fusion. (A) A typical record from an RBL cell. The Ca 2+ -activated conductance rise was used to define the t 0 point. (B) The rise of capacitance was well described by the delayed monoexponential function given in the figure. The variable k 2 is the rate constant used for statistical analysis. The number of the data points was reduced to highlight the fitted curve. Antibodies against PI(3)P (C), PI(4)P (D), and both (E) fail to block non-SG fusion.

    Journal: The Journal of General Physiology

    Article Title: Ca-dependent Nonsecretory Vesicle Fusion in a Secretory Cell

    doi: 10.1085/jgp.200709950

    Figure Lengend Snippet: Non-SG fusion is not blocked by antibodies against PI(3)P and PI(4)P. In whole-cell recording, antibodies (1:100 dilution) were added to the cytoplasmic (pipette) solution, which was dialyzed into cells for 5 min before Ca 2+ was infused to trigger fusion. (A) A typical record from an RBL cell. The Ca 2+ -activated conductance rise was used to define the t 0 point. (B) The rise of capacitance was well described by the delayed monoexponential function given in the figure. The variable k 2 is the rate constant used for statistical analysis. The number of the data points was reduced to highlight the fitted curve. Antibodies against PI(3)P (C), PI(4)P (D), and both (E) fail to block non-SG fusion.

    Article Snippet: Capacitance measurement software, Capmeter 6, was developed in MATLAB using its data acquisition toolbox (R2006b; The MathWorks).

    Techniques: Transferring, Blocking Assay